关键词: 花背蟾蜍蝌蚪, 角膜发育, Ⅰ型胶原, 免疫组化, 图像分析

Abstract: Using the technology of immunohistochemical staining and color image analysis to investigate the spatiotemporal pattern and age_related changes of type Ⅰ collagen expression in the corneal development of Bufo raddei larvae during metamorphosis.Results showed that the inner and outer cornea combined together into mature cornea during metamorphosis.Both corneal epithelium and corneal stroma become thickened with progressively increase of the age.The analysis of immunohistochemical staining by color image analysis found that type Ⅰcollagen expressed widely during the period,existing in Bowman's membrane,Descemet's membrane,corneal stroma and endothelium.Espeacially,a progressively change in corneal stroma was observed.ConclusionThe defect of qualitative index may be remedied by color index in the quantitative immunohistochemical image analysis of type Ⅰ collagen.The inner and outer cornea combine together gradually with type Ⅰ collagen increasing in the corneal development of Bufo raddei larvae during metamorphosis.Type Ⅰcollagen,which is the main component in the increased collagens,expresses progressively with age.Type Ⅰcollagen may be the important component in corneal stroma,and it plays an important role in the construction and maintaining of the corneal stroma.

Key words: Bufo raddei larvae, Cornea development, Type Ⅰ collagen, Immunohistochemistry, Image analysis