猪细小病毒血清学检测方法的建立

实验动物科学

猪细小病毒血清学检测方法的建立

吴淑芳;付瑞;邢瑞昌;贺争鸣;

中国药品生物制品检定所国家实验动物微生物遗传检测中心,中国药品生物制品检定所国家实验动物微生物遗传检测中心,中国药品生物制品检定所国家实验动物微生物遗传检测中心,中国药品生物制品检定所国家实验动物微生物遗传检测中心北京100050,北京100050,北京100050,北京100050

收稿日期:2007-04-28 修回日期:2007-04-28 出版日期:2007-04-28 发布日期:2007-04-28

Establishment and Application of Serological Test for Porcine Parvovirus

WU Shu-fang,FU Rui,XING Rui-chang,HE Zheng-ming

(National Institute for the Control of Pharmaceutical and Biological Products, National Center for Microbiological and Genetic Monitoring of Laboratory Animal,Beijing 100050

Received:2007-04-28 Revised:2007-04-28 Online:2007-04-28 Published:2007-04-28

摘要/Abstract

摘要： 目的建立猪细小病毒血清学检测方法。方法用抗猪细小病毒的猪源性血清,通过IFA和IEA两种方法,首先将阳性血清吸附PK-15细胞抗原,以排除非特异反应,通过对阳性血清及3种二抗(其中IFA以FITC标记的抗猪IgG抗体,IEA分别以HRP标记的抗猪IgG抗体和SPA-HRP作为二抗)进行浓度梯度滴定,确定最佳工作浓度,并比较三种反应体系的敏感性。结果IFA最高能检测出PPV-猪组织混合感染PK-15细胞模型中1个TCID50,而其它两种反应体系均只能检测100个TCID50。结论间接免疫荧光检测方法比其它两种方法敏感性更高。

关键词: 猪细小病毒, 间接免疫荧光, 免疫酶

Abstract: ObjectiveTo establish the serological method for detection of porcine parvovirus(PPV).MethodsFirstly,the swine serum containing the antibodies against PPV was absorbed by the PK-15 to eliminate nonspecific reaction,next,the optimal reaction was determined by concentration gradient titration of the serum and the second reaction-antibodies,and then,the sensitivity among the reaction systems was compared.Results 1 TCID_50 could be acquired with IFA,however,only 100 TCID_50 could be acquired with the other two methods. ConclusionIFA method was more sensitive than the other two methods.

Key words: Porcine parvovirus, IFA, IEA

吴淑芳;付瑞;邢瑞昌;贺争鸣;. 猪细小病毒血清学检测方法的建立[J]. 实验动物科学.

WU Shu-fang,FU Rui,XING Rui-chang,HE Zheng-ming. Establishment and Application of Serological Test for Porcine Parvovirus[J]. laboratory animal science.

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