关键词: 24h尿渗透压, 动物利尿筛选, 利尿药理实验, 中草药

Abstract: Objective To delve into the method of screening the rabbits suited to diuretic pharmacological experiment on Chinese medicinal herbs and determine the key factors.Methods The rabbits were placed in the metabolic cages ad lib food and drink and 100 mL distilled water was orally administered to every rabbit 4 hours after taking food once every day so as to adjust urine osmotic pressure.24-hour water-intake was measured once every day.24-hour urine was collected and 24-hour urine volume was measured.The osmotic pressure,Na concentration,and K concentration of 24-hour urine were determined.Results The osmotic pressure of 24-hour urine was negatively correlated with 24-hour water-intake and 24-hour urine volume,and was positively correlated with Na concentration and K concentration of 24-hour urine.The osmotic pressure of 24-hour urine was 200～800 mOsm/L,and the relative 24-hour water-intake,24-hour urine volume,Na concentration,and K concentration of 24-hour urine were within the physiological range.Conclusion The osmotic pressure of 24-hour urine is key factor of the screening method.24-hour urine volume,Na concentration,and K concentration of 24-hour urine can be stabilized within the physiological range by adjusting urine osmotic pressure,which enables the animal to micturate homogeneously.

Key words: Osmotic pressure of 24-hour urine, Diuretic pharmacological experiment, Chinese medicinal herbs