关键词: 巴马小型猪, 初情期

Abstract: The objectives of this study were to examine the prepuberty and semen characteristics of male Guangxi Bama minipig.Testes of 15,20,25,26,27, 28,29,30,45,55,62,75 days old Bama minipig were formalin-fixed and paraffin-embedded and stained with hematoxylin and eosin,and were examined if sperms exist.Semen of Bama minipig were collected and sent to Guangxi Center for analysis and test research to analyze its physical characteristics and chemical compositions.The results showed that:(1) the prepubertal age of male Bama minipig is preliminarily determined to be 45 days old approximately;(2)the physical characteristics(ejaculate volume 107.5 mL±39.0 mL,pH 7.6±0.3,osmolarity 316±3 mOsm,sperm viability 0.754±0.079,motility 0.376±0.168,abnormality 0.256±0.159,concentration 73.2×10~6/mL) and chemical compositions(DNA 127.4 μg±8.0 μg/mL,protein 6.0 g±0.7 g/100 mL,phospholipids 8.7 mg±0.2 mg/100 mL,fructose 117.4 mg±81.0 mg/100 mL,prostaglandin 3.6 pg±0.8 pg/ mL,citric acid 45.6 mg±77.6 mg/100 mL,Na~+ 1.56 mg±0.13 mg/mL,K~+ 0.78 mg±0.06 mg/mL,Cl~-1.62 mg±0.18 mg/mL)of Bama minipig semen were analyzed.

Key words: Bama minipig, Prepuberty, Semen