关键词: 小型猪, Ⅱ型糖尿病, 动物模型, 五指山小型猪

Abstract: Objective To establish and compare the typeⅡdiabetes mellitus models in three strains of native minipigs for promoting their use on type Ⅱdiabetes mellitus research.Methods Six Bama minipigs,six Wuzhishan minipigs and six Nongda minipigs were fed with a high-sucrose high-fat diet to induce type Ⅱ diabetes mellitus.Four Nongda minipigs were assigned as controls fed with a normal diet.Body weight,fasting serum glucose and insulin concentrations were measured monthly,and serum TG,CHO,HDL-C,LDL-C and intravenous glucose tolerance test(IVGTT) were tested at 2 month intervals during the 8-month inducing period.Results Bama minipigs and Wuzhishan minipigs were susceptible to the high-sucrose high-fat diet.Obesity,hyperinsulinemia and impaired insulin tolerance were noted at month 5 and 6.One of six Bama minipigs and one of six Wuzhishan minipigs developed diabetes mellitus and three Bama minipigs and two Wuzhishan minipigs reached the pre-diabetes stage at month 8.Nongda minipigs were relatively resistant to the high-sucrose high-fat diet.Fasting serum glucose,insulin concentration,insulin sensitivity index and IVGTT remained normal during the 8 months high-sucrose high-fat feeding.Only slightly obesity and despaired insulin tolerance were observed at month 7 and 8.All the parameters related to blood lipid metabolism(TG,CHO,HDL-C,LDL-C) were increased significantly after feeding high-sucrose and high-fat in the three strains of minipigs with Bama minipigs increased more significantly.Conclusion Bama minipigs and Wuzhishan minipigs were susceptible to the high-sucrose and high-fat diet inducing typeⅡdiabetes millitus.Susceptibility differences existed among the animals in the same strains.Nongda minipigs were less susceptible to the dietary inducing.The results provided foundamental data for further research on typeⅡdiabetes mellitus susceptibility relative genes in the minipigs and for selective breeding of family stocks with high typeⅡdiabetes mellitus susceptibility.

Key words: Minipig, TypeⅡdiabetes mellitus, Animal model, Bama minipig, Wuzhishan minipig