关键词: 传染性支气管炎病毒, 血凝抗原

Abstract: Objective To prepare IBV agglutination antigen via treating with phospholipase C.Methods IBV was inoculated into allantoic cavity of 9-day-old SPF chicken embryo.The eggs were incubated at 37℃ for 48 hours.Allantoic fluid was collected and centrifuged at high speed for concentration,following to individually treated with trypsin and clostridium perfringen culture(containing phospholipase C).Then performing routine agglutination test and agglutination inhibition test.Results IBV haemagglutination showed strong non-specificity by means of trypsin treatment.On the contrary,IBV haemagglutination expressed powerful specific with phospholipase C because the haemagglutination could be inhibited by specific antiserum to IBV,simultaneously could not be inhibited by antiserum to ILTV,NDV,Adeno-1 and 3,APIV,AIV,IBDV,HP,MS and MG.Conclusion IBV treated with phospholipase C could be used for haemagglutination inhibition test.

Key words: Infectious Bronchitis Virus(IBV), Haemagglutination antigen