套式PCR检测淋巴细胞脉络丛脑膜炎病毒(LCMV)方法应用

实验动物科学

套式PCR检测淋巴细胞脉络丛脑膜炎病毒(LCMV)方法应用

邢进;肖镜;王吉;贺争鸣;

中国药品生物制品检定所实验动物质量检测室;

收稿日期:2008-10-28 修回日期:2008-10-28 出版日期:2008-10-28 发布日期:2008-10-28

Application of Assay for The Detection of LCMV Nested Polymerase Chain Reaction

XING Jin,XIAO Jing,WANG Ji,HE Zheng-ming

(Department of The Quality of Laboratory Animal Testing,National Institute for The Controlof Pharmaceutical & Biological Products,Beijing 100050,China

Received:2008-10-28 Revised:2008-10-28 Online:2008-10-28 Published:2008-10-28

摘要/Abstract

摘要： 目的检测目前小鼠和地鼠中淋巴细胞脉络丛脑膜炎病毒的感染情况。方法采用套式PCR方法对小鼠和地鼠脑及小鼠脏器进行扩增,检测LCMV基因;同时对血清进行ELISA试验,将两种方法的结果进行比较。结果从小鼠的脾脏和肾脏中检测到NP基因的存在,检出率2/148;ELISA检出率5/148。结论PCR与ELISA检测方法相结合,准确率高,证明目前小鼠中存在LCMV的感染。

关键词: 淋巴细胞脉络丛脑膜炎病毒, 套式PCR检测

Abstract: Objective Monitoring infection of Lymphocytic Choriomeningitis Virus in mice and Golden hamster currently.Methods Using nested-PCR method in mice and Golden hamster to detect the presence of LCMV gene.At the same time to take the serum ELISA test,the results of the two methods were compared.Results[WTBZ] The PCR results showed existence of NP gene in spleen and kidney of mice.PCR positive rate is 2/148,ELISA is 5/148.Conclusion Combining of PCR and ELISA method can improve accuracy rate and prove currently exist in mice LCMV infection.

Key words: LCMV, Nested-PCR test

邢进;肖镜;王吉;贺争鸣;. 套式PCR检测淋巴细胞脉络丛脑膜炎病毒(LCMV)方法应用[J]. 实验动物科学.

XING Jin,XIAO Jing,WANG Ji,HE Zheng-ming. Application of Assay for The Detection of LCMV Nested Polymerase Chain Reaction[J]. laboratory animal science.

[1]	佟巍;乔红伟;魏强;张丽芳;代小伟;刘先菊;王艳荣;刘云波;. 淋巴细胞脉络丛脑膜炎病毒在实验大鼠中的感染状况[J]. 实验动物科学, 2010, 27(04): 46-48.
[2]	邢进;卫礼;巩薇;贺争鸣;邢瑞昌;. 淋巴细胞脉络丛脑膜炎病毒(LCMV)PCR检测方法的建立与初步应用[J]. 实验动物科学, 2006, 23(03): 5-8.