关键词: 树突状细胞, 放疗, 肾癌, 瘤内注射

Abstract: Objective To investigate the effects of intratumoral dendritic cells injection combined with radiotherapy on cytokines in the mice with renal carcinoma.Methods[WTBZ] BALB/c mice received renal cancer cell(RENCA) vaccination(subcutaneous injection of 2.5×10~6 RENCA cells),and then radiotherapy(7 Gy/time) and intratumoral injection of unpulsed dendritic cells at 1×10~6 cells/mouse/time were carried out.The mice were sacrificed on twenty-eighth day.Growth rate,weight and size of tumor mass were measured,and IL-2,IFN-γ,IL-4,IL-10,and TNF-α levels in splenocytes were determined by ELISA assays.Results Growth of tumor mass in the group of intratumoral dendritic cells injection combined with radiotherapy was significantly slower than that in other groups,and IL-2,IFN-γ and IL-4 levels in splenocytes in the group of intratumoral dendritic cells injection combined with radiotherapy was significantly higher than those in other groups.Conclusion Intratumoral dendritic cells injection combined with radiotherapy can inhibit growth tumor in the mice.

Key words: Dendritic cell, Radiotherapy, Renal Carcinoma, Intratumoral Injection