关键词: 卷烟, 仅鼻吸烟, 咳嗽, 痰液分泌, 动物模型

Abstract: Objective To establish a cigarette induced cough and sputum secretion mice model for effective evaluation of the effect of reduce the dangers of cigarettes with additives.Methods Mice were exposed to smoke through the nose-only device for one hour everyday,the cough latency time and cough frequency were tested by ammonia water method and the OD values of sputum secretions was tested by phenol red method.Results The cough time of model group mice significantly decreased,the cough frequency of model group mice increased,the OD values of sputum secretions of model group mice significantly increased.Conclusion It is successful to establish a cigarette induced cough and sputum secretion mice model.

Key words: Cigarette, Nose-only, Cough, Sputum secretion, Animal model