建立Ⅱ型糖尿病大鼠模型的探讨

实验动物科学

建立Ⅱ型糖尿病大鼠模型的探讨

董建一;王亮;王福金;李慧玲;詹红微;王靖宇;

大连医科大学实验动物中心;

收稿日期:2010-08-28 修回日期:2010-08-28 出版日期:2010-08-28 发布日期:2010-08-28

Exploration of Establishing ⅡDiabetes Mellitus Rats Model by Complex Methods

DONG Jian-yi,WANG Liang,WANG Fu-jin,LI Hui-ling,ZHAN Hong-wei,WANG Jing-yu

(Laboratory Animal Center,Dalian Medical University,Dalian 116044,China

Received:2010-08-28 Revised:2010-08-28 Online:2010-08-28 Published:2010-08-28

摘要/Abstract

摘要： 目的探讨Ⅱ型糖尿病SD大鼠模型的稳定性与耐受性。方法采用连续4周喂养高脂饲料后,空腹一次性注射25 mg/kg链脲佐菌素的方法,建立Ⅱ型糖尿病SD大鼠模型。结果造模7 d后血糖值均高于6.78 mmol/L。持续观察22 d,血糖值后期略有波动,但稳定性与耐受性良好(均高于6.78 mmol/L)。结论采用高脂饮食,联合空腹一次性注射25 mg/kg链脲佐菌素的方法能成功建立Ⅱ型糖尿病SD大鼠模型。该方法建立模型简单、用药量小,有良好的稳定性与耐受性。

关键词: Ⅱ型糖尿病, SD大鼠模型, 稳定性, 耐受性

Abstract: Objective To observe the effect of stability and tolerance of the Ⅱdiabetes mellitus SD rats model.Methods SD rats are injected intraperitoneally with 25 mg /kg streptozotocin once after fed high fat diet 4 weeks.Results when the SD rats were injected STZ one week,the blood glucose of SD rats were stably higher then 6.78 mmol /L.During the 22 days observation,Although the blood glucose values of SD rats have fluctuated at a latter stage,the stability and tolerated of blood glucose of SD rats were well,were over 6.78 mmol /L.Conclusion The Ⅱdiabetes rats model can be established successfully with feeding high fat diet and injecting a low dose STZ(25 mg/kg) once.This method is simple and use a small amount of medication,the model has hatter stability and tolerance.

Key words: Ⅱ Diabetes mellitus, SD rats model, Stability and Tolerance

董建一;王亮;王福金;李慧玲;詹红微;王靖宇;. 建立Ⅱ型糖尿病大鼠模型的探讨[J]. 实验动物科学.

DONG Jian-yi,WANG Liang,WANG Fu-jin,LI Hui-ling,ZHAN Hong-wei,WANG Jing-yu. Exploration of Establishing ⅡDiabetes Mellitus Rats Model by Complex Methods[J]. laboratory animal science.

[1]	魏杰，王洪，贾松华，岳秉飞. 过氧化氢酶-2能力验证样品的制备及其均一性和稳定性测定[J]. 实验动物科学, 2019, 36(04): 39-.
[2]	王洪，魏杰，邢进，巩薇，岳秉飞. 用五种标准物质的稳定性评价实验动物遗传质量研究[J]. 实验动物科学, 2018, 35(06): 32-.
[3]	龚菁菁，李小红，王存龙，李银银，陈振文，杜小燕. Nf-κb在遗传性糖尿病长爪沙鼠多种组织中的表达状况[J]. 实验动物科学, 2018, 35(02): 33-.
[4]	赵玉琼;陈华;. PGC-1基因与Ⅱ型糖尿病[J]. 实验动物科学, 2008, 25(06): 45-47.
[5]	陈华;刘亚千;李春海;郭雄明;黄丽洁;. 三个品系小型猪Ⅱ型糖尿病模型的比较研究[J]. 实验动物科学, 2007, 24(06): 49-55.
[6]	杜颖,黄清泉,贺争鸣,李冠民. 家兔对细菌内毒素致热耐受性的实验研究[J]. 实验动物科学, 2003, 20(04): 13-15.
[7]	刘光泽,贾彦征,王洪敏,熊一力. 乙型肝炎病毒转基因小鼠外源基因的复制和传代稳定性观察[J]. 实验动物科学, 2001, 18(01): 1-3.