关键词: iRhom2^Uncv, 皮肤损伤, 小鼠, 代谢组

Abstract: Objective　To explore the differential metabolites and metabolic pathways related to skin injury repair in iRhom2^Uncv, and to provide a basis for in-depth study of potential biomarkers and targets for skin injury repair in iRhom2^Uncv.Methods　Thirty-two SPF-grade adult male iRhom2^Uncv and BALB/c mice were randomly divided into four groups. A full-thickness skin wound model was established under anesthesia. The skin tissues were analyzed with immunohistochemistry at 0, 5 and 12 days post injury. The serum was collected for metabolite analysis.Results　The wound healing rate of iRhom2^Uncv was slightly higher than that of BALB/c. The inflammatory response of iRhom2^Uncv was higher, and the number of new blood vessels at 12 days post-injury was higher than that of wild-type BALB/c mice. Differential metabolites were significantly enriched in six metabolic pathways, including arachidonic acid metabolism, regulation of inflammatory mediators in TRP channels, histidine metabolism and thyroid hormone synthesis.Conclusion　The ability of iRhom2^Uncv in skin wound healing is stronger than that of BALB/c, and the affected metabolites and metabolic pathways can provide a basis for further research of the metabolic mechanism related to skin wound repair in iRhom2^Uncv.

Key words: iRhom2^Uncv, skin wound, mouse, metabolome