关键词: 食蟹猴, 盐酸氯胺酮, 戊巴比妥钠, 丙泊酚, 麻醉剂

Abstract: Objective　To systematically compare the efficacy and safety of different anesthetic protocols in Cynomolgus monkeys for optimizing anesthesia management in primate research.Methods　Seventy two monkeys were stratified by age into juvenile (1-6 years) and senior (12-17 years) groups, receiving one of three anesthetic regimens: (1) intravenous 3% pentobarbital sodium; (2) intramuscular ketamine hydrochloride (10 mg/kg); or (3) ketamine induction followed by propofol infusion. Comprehensive monitoring included anesthesia induction time, maintenance duration, core physiological parameters (core temperature, heart rate, respiratory rate), and serum biochemical markers (AST, ALT, CK). Results　Pentobarbital sodium demonstrated optimal performance in juvenile subjects, characterized by rapid induction period (≤2 min) and prolonged duration (≥100 min), whereas senior animals exhibited delayed induction period and reduced efficacy, necessitating dose adjustment. Ketamine monotherapy provided stable short-duration anesthesia (10-20 min) with minimal physiological perturbations. The ketamine-propofol combination proved effective for extended procedures but required careful monitoring of thermoregulation and postoperative biochemical alterations, particularly significant CK elevation.Conclusion　This study reveals age-dependent anesthetic responses in M. fascicularis, establishing evidence-based guidelines for protocol selection in nonhuman primate research to enhance experimental standardization and animal welfare.

Key words: Cynomolgus monkeys, ketamine hydrochloride, sodium pentobarbital, propofol, anesthetic agents