关键词: 免疫缺陷大鼠, 血液学, 淋巴细胞亚群, 脏器系数

Abstract: Objective The study aimed to detect and analyze the main biological characteristics of domestic commercial immunodeficient rats, including the percentage of blood lymphocyte subsets, histology of immune organs, organ coefficients, and blood physiological indexes. Method Six strains of immunodeficient rats of three categories commercially available in China were selected, as well as corresponding control with the same genetic background. The blood physiological indexes, blood lymphocyte subsets, organ coefficients of major immune organs, and histological features were detected and compared. Result ( 1) The thymus coefficient and histological features of T lymphocyte-deficient rats showed significant differences compared to the control group (P<0. 01) . The levels of WBC (white blood cells) , LYMPH ( lymphocytes) , LYMPH% ( lymphocyte percentage) , as well as the proportions of T cell, were significantly lower than those in the control group ( P<0. 01) . In contrast, the levels of NEUT ( neutrophils) , NEUT% ( neutrophil percentage) , the proportions of B cell, and the proportions of NK ( natural killer) cell were higher than those in the control group ( P<0. 01) . Significant differences (P<0. 01) were also observed in the spleen coefficient, WBC, LYMPH, LYMPH%, NEUT%, and the proportions of B cell among immunodeficient rats from different manufacturers. (2) The spleen coefficient and thymus coefficient of rats with combined T and B lymphocyte deficiency, as well as their histological features, showed significant differences compared to the control group ( P<0. 01) . The levels of WBC, LYMPH, NEUT %, the proportions of T cell, and the proportions of B cell were all significantly lower than those in the normal control group (P<0. 01) . In contrast, the percentages of MONO (monocytes) , NEUT, NEUT %, and the proportions of NK cell were significantly higher than those in the control group (P<0. 01) . (3) The spleen coefficient and thymus coefficient of rats with combined T, B, and NK cell deficiency were significantly lower than those of the control group ( P < 0. 05,P < 0. 01) . The levels of WBC, LYMPH, LYMPH % as well as the proportions of T cell and B cell, were all significantly lower than those of the control group (P<0. 01) . The percentages of NEUT and MONO were higher than those of the control group (P<0. 01) . Significant differences were observed in the LYMPH %, NEUT%, and MONO% among rats with combined T, B, and NK cell deficiency from different manufacturers ( P < 0. 01) . Conclusion The immune cell counts, histology of immune organs, and hematology indexes of 6 strains animals generally conform to the main biological characteristics of immunodeficient rats. However, certain lymphocyte subsets, blood physiological indexes, and organ coefficients are various in immunodeficient rats of the same strain from different manufacturers. The present study provides an important basis for establishment of criteria and application of standardized management for immunodeficient rats.

Key words: immunodeficient rats, hematology, lymphocyte subsets, organ coefficients