关键词: 高压灭菌水, 酸化水, 无菌水袋, SPF 小鼠, 生长发育

Abstract: Objective To compare the effects of three different drinking water treatments on the growth and development of SPF mice. Method 54 SPF mice were randomly divided into 3 groups. They were fed with autoclaved water, autoclaved acidified water and sterile water bag water for 90 days. Other feeding conditions were the same. During the feeding process, the routine water quality indexes, microorganisms, body weight, drinking water volume, organ weight and organ pathomorphology in drinking water were measured, and the data were analyzed by SPASS 13. 0. Result The routine indexes of drinking water quality were qualified. The sterility test of sterile water bag water and autoclaved acidified water was negative within 14 days, and the test was positive from the first day of autoclaved water; within 14 days, the daily water intake of autoclaved acidified water group was significantly lower than that of autoclaved water group ( P < 0. 01) ; compared with the autoclaved water group, the heart coefficient of the water bag group was significantly higher, the lung index was significantly lower ( P < 0. 05) , and the liver coefficient of the acidified water group was significantly higher ( P < 0. 05) ; the result of histopathological morphology showed that the organs of mice were normal without pathological changes. Conclusion Autoclaved acidified water and sterile water bag water are better than autoclaved water in bacteriostatic aspects, and sterile water bag water and autoclaved water are better than autoclaved acidified water in drinking water volume. The effects of drinking water with three different treatment method on organs and body weight were not statistically significant.

Key words: autoclaved high pressure, acidified water, sterile water bag water, SPF mice, growth and development