关键词: 针刺, 骨质疏松症, 青年期大鼠, micro-CT

Abstract: Objective To observe the effects of acupuncture on bone metabolism and bone quality in ovariectomized osteoporosis model rats, and to explore the mechanism of acupuncture in treating osteoporosis in youth. Method Forty female SD rats were randomly divided into a normal group, a sham operation group, a model group and a acupuncture group, with 10 rats in each group. The model group and acupuncture group used the ovary to prepare the rat model of osteoporosis in youth. The model group did not carry out intervention treatment. The acupuncture group was given electroacupuncture to stimulate “ Shenshu” , “ Zusanli” and “Xuanzhong” at the 2nd week after operation, once a day, for 8 consecutive weeks. The contents of serum alkaline phosphatase ( ALP ) and osteocalcin ( OC) were detected by enzyme-linked immunosorbent assay ( ELISA ) ; Femoral bone mineral density ( BMD ) and bone morphological parameters were measured by micro CT. Result After 8 weeks of intervention, compared with the normal group and sham operation groups, ALP and OC increased in the model and acupuncture groups, BMD, BV / TV and Tb. Th decreases,and Tb. Sp increased (P < 0. 05) , indicating the success of osteoporosis modeling. Compared with the model group, ALP and OC in the acupuncture group decreased, BMD、 BV / TV and Tb. Th increases, and Tb. Sp decreased ( P < 0. 05 ) , indicating that acupuncture can improve bone mineral density and bone microstructure. Conclusion Acupuncture has the effect of preventing osteoporosis in youth, and its mechanism may be related to the promotion of bone formation and repair, the increase of bone density, and the enhancement of the structural strength of bone trabeculae.

Key words: acupuncture, osteoporosis, youth rats, micro-CT