关键词: 实验动物, 脾, 白髓细胞减少, 髓外造血

Abstract: Objective In order to provide suggestions for future laboratory animal inspection and feeding, we observed the pathological changes of spleen in rodent laboratory animals in Beijing in October 2020, and analyzed the possible causes of pathological changes. Method After euthanasia, the spleens of the animals were taken and sectioned for HE staining. Pathological observation and diagnosis were made by light microscope. Result A total of 360 rodents ( including 170 mice, 80 rats, 100 guinea pigs and 10 hamsters) were tested. Among them, 10 SPF ICR mice and 10 SPF SD rats from different institutions showed pathological changes, such as the decrease of white pulp cells, the increase of extramedullary hematopoiesis and macrophages of red pulp, and so on. Conclusion The pathological changes of spleen in sampling examination may be related to the age of laboratory animals, long-term exposure to certain toxic substances, autoimmune deficiency, infection or tumors. Therefore, in the sampling inspection of the quality of laboratory animals, institutions should avoid sending young or old animals for inspection, and at the same time further improve the feeding and management system to ensure the health of laboratory animals.

Key words: laboratory animals, spleen, white pulp cellularity decrease, extramedullary hematopoiesis