Abstract: Objective To provide reference to production, management and detection of laboratory animals by comparative analyzing of the recently 3 years’ test result from 2017 to 2019 on microbes and genetics of laboratory animals in Beijing area. Method According to the national and local standards, relevant law and regulation, the laboratory animal production units in Beijing area were randomly inspected and the tested. According to the data, 8 species of the laboratory animals including mice, rats, guineas, shrews, rabbits, dogs, monkeys and minipigs were classified according to their breeds, grades, units, etc. , and their quality problems were analyzed. The change in trend analysis of the quality of the laboratory then was analyzed and compared with the same period from 2014 to 2016. Result From 2017 to 2019, there were 144, 131 and 135 batches of random testing annually, while 30, 15 and 17 unqualified batches, respectively. The main reasons for disqualification were the immunity level dissatisfaction (20 batches in rabbits, dogs and minipigs) and 20 batches were infected by pathogens. No genetic variation was found. Compared with the relevant results from 2014 to 2016, no genetic problems have been detected. The status of substandard immunity has improved and pathogenic infections have increased. Conclusion This result can provide support to the healthy development of the laboratory animal industry.