氢吗啡酮对炎性痛大鼠脊髓 ERK1 / 2 信号通路的影响

实验动物科学 ›› 2021, Vol. 38 ›› Issue (3): 34-.

氢吗啡酮对炎性痛大鼠脊髓 ERK1 / 2 信号通路的影响

(湖北省黄石市中心医院普爱院区麻醉科,黄石 435001)

出版日期:2021-08-06 发布日期:2021-08-23

Effect of Hydromorphine on ERK1 / 2 Signal Pathway in Spinal Cord of Rats with Inflammatory Pain

( Department of Anesthesiology, Puai District, Huangshi Central Hospital, Hubei Province, Huangshi 435001, China)

Online:2021-08-06 Published:2021-08-23

摘要/Abstract

摘要：

摘要:目的探讨氢吗啡酮对炎性痛大鼠脊髓 ERK1 / 2 信号通路的影响。方法选择 SPF 级雄性大鼠 30 只,随机分为 3 组,正常组、模型组和氢吗啡酮组;检测各组大鼠热痛阈和机械痛阈;ELISA 检测血清中 TNF-α、IL-6 和 IL-10的含量;Western blot 检测各组大鼠脊髓中 ERK1 / 2 和 p-ERK1 / 2 蛋白表达水平;qRT-PCR 法检测各组大鼠脊髓中ERK1 / 2 mRNA 表达水平。结果与正常组相比,模型组大鼠血清炎症细胞因子 TNF-α 和 IL-6 含量明显增高,IL-10 含量明显降低,且术侧后肢热痛阈显著缩短及机械痛域明显降低,差异均有统计学意义 ( P < 0. 05) ;脊髓中ERK1 / 2 mRNA 表达水平、ERK1 / 2 及 p-ERK1 / 2 蛋白表达水平均显著升高,差异有统计学意义( P< 0. 05) 。注射氢吗啡酮后,与模型组相比,大鼠血清 TNF-α 和 IL-6 含量显著降低而 IL-10 含量显著升高,热痛阈显著延长及机械痛域明显升高,差异均具有统计学意义( P<0. 05) ;氢吗啡酮组 ERK1 / 2 mRNA 表达水平、ERK1 / 2 和 p-ERK1 / 2 蛋白表达水平均明显降低( P<0. 05) 。结论氢吗啡酮可有效改善弗氏完全佐剂诱导的大鼠炎性疼痛,其抗炎镇痛机制与降低大鼠机体炎性因子的产生,并抑制 ERK1 / 2 信号通路的活化有关。

Abstract:

Abstract: Objective To investigate the effect of hydromorphine on ERK1 / 2 signal pathway in spinal cord of rats with inflammatory pain. Method Thirty SPF male rats were randomly divided into three groups: the normal group, the model group and the hydromorphine group. Detection of thermal pain threshold and mechanical pain threshold were detected in rats of each group. TNF-α, IL-6 and IL-10 in serum were tested by ELISA. Western blot detected the ERK1 / 2 and p-ERK1 / 2 protein expression in spinal cord of rats in each group. The expression of ERK1 / 2 mRNA in spinal cord of rats in each group was detected by qRT-PCR method . Result Compared with the normal group, the content of serum inflammatory cytokines TNF-α and IL-6 in the model group was significantly higher than that in the normal group, while the content of IL-10 in the model group was significantly lower than that

in the normal group, and the thermal pain threshold was significantly shortened and the mechanical pain domain was significantly decreased in the model group ( P<0. 05) . The expression levels of ERK1 / 2 mRNA, ERK1 / 2 and p-ERK1 / 2 protein in spinal cord of the model group were significantly higher than those of the normal group ( P< 0. 05) . After injection of hydromorphine, compared with the model group, the contents of serum TNF-α and IL-6 were significantly decreased, while the content of IL-10 was significantly increased, the thermal pain threshold was significantly prolonged and the mechanical pain domain was significantly increased ( P<0. 05) . The expression of ERK1 / 2 mRNA, ERK1 / 2 and p-ERK1 / 2 protein decreased significantly in hydromorphine group ( P < 0. 05 ) .Conclusion Hydromorphine can effectively improve complete freund ’ s adjuvant-induced inflammatory pain in rats. Its anti-inflammatory and analgesic mechanism is related to its reduction of the production of inflammatory factors and inhibition of the activation of ERK1 / 2 signal pathway in rats.

石晓玲, 贾伟. 氢吗啡酮对炎性痛大鼠脊髓 ERK1 / 2 信号通路的影响 [J]. 实验动物科学, 2021, 38(3): 34-.

SHI Xiaoling, JIA Wei. Effect of Hydromorphine on ERK1 / 2 Signal Pathway in Spinal Cord of Rats with Inflammatory Pain [J]. Laboratory Animal Science, 2021, 38(3): 34-.

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