关键词: <, p>, 中药, 斑马鱼, 肝脏毒性<, /p>

Abstract: Abstract: Objective To determine the hepatotoxicity of 5 known mammalian hepatotoxic drugs ( Zhuangguguanjie ［ZGGJ］pill，Yi Shen Wu Fa oral liquid，Niuhuang Jiedu Pian，Tripterygium Glycosides ［TWP］，saikosaponin d) in larval zebrafish and to validate the practicability of zebrafish model for rapid assessment of drug hepatotoxicity． Method Zebrafish at 3 dpf ( days past fertilization) were treated with Chinese medicines at series of concentration for 2 days． At the end of treatment，zebrafish from each group were randomly selected and images were acquired under a fluorescent stereomicroscope and quantitatively assessed in larval zebrafish using three specific phenotypic endpoints of hepatotoxicity: liver degeneration，changes in liver size and yolk sac retention． Zebrafish liver degeneration was originally screened visually，quantified using an image-based morphometric analysis． Ｒesult ZCGJ pill induced liver degeneration and reduced liver size in larval zebrafish，Yi Shen Wu Fa oral liquid induced liver degeneration and increased liver size in larval zebrafish，Niuhuang Jiedu Pian reduced liver size in larval zebrafish，TWP and saikosaponin d increased liver size in larval zebrafish． All the tested mammalian hepatotoxic drugs delayed yolk sac absorption in larval zebrafish． Conclusion Chinese medicines could induce the liver damage in larval zebrafish． Zebrafish phenotypic assay is a highly predictive animal model for rapidly in vivo assessment of compound hepatotoxicity．

Key words: <p>Chinese medicines, Zebrafish, hepatotoxicity</p>