关键词: H22 肝癌, 实体瘤, 免疫功能指标, 环磷酰胺

Abstract: Abstract: Objective Standardized determination of immune function indicators and effect of cyclophosphamide on the H22 solid tumors in mice． Method H22 cells were implanted into the left axilla of 46 BALB /c mice． Tumors growth was observed 1 days later，The mice were randomly divided into tumor-bearing mice + cyclophosphamide group，tumor-bearing mice group，control mice + cyclophosphamide group，control mice group． Cyclophosphamide group intraperitoneal injection of cyclophosphamide 50mg /kg，2 times a week． Observe the general clinical manifestations，tumorigenicity，body weight，food intake，tumor volume，tumor weight，immune organ weight， blood values，blood biochemistry values，cytokines，lymphocyte transformation，NK cell activity，lymphocyte subsets and histopathology． Result Compared with normal mice，H22 tumor-bearing mice reduced food consumption，RBC，HGB，HCT significantly decreased ( P ＜ 0. 01) ，WBC，PLT，RDW significantly increased ( P ＜ 0. 01) ，GOT，GPT significantlyincreased ( P ＜ 0. 01) ，CD4 － CD8 － significantly decreased ( P ＜ 0. 01) ． The average food intake reduces the tumor-bearing mice given cyclophosphamide，D9 to D14 body weight was significantly lower ( P ＜ 0. 05) ，D5 to D14 tumor volume was significantly lower ( P ＜ 0. 01) ，tumor weight，spleen weight，thymus weight was significantly sexual lowering ( P ＜ 0. 01) ，the WBC，MCV significantly with lowering ( P ＜ 0. 01 ) ，HGB，the MCH，MCHC，the PLT significantly with increased ( P ＜ 0. 05 ) ，the GOT，GPT significantly ( P ＜ 0. 01) lower of IL － 2 significantly with reducing ( P ＜ 0. 01) ，IFN － γ significantly increased ( P ＜ 0. 01 ) ，CD4 + CD8 － ( %) ，CD4 + CD8 + ( %) ，CD4 － CD8 + ( %) ，CD3 + ( %) lymphocytes significantly with increased ( P ＜ 0. 01) ，CD4 － CD8 － ( %) lymphocytes was significantly lower ( P ＜ 0. 01) ，spleen lymphocytes transformed absorbance difference significantly lower ( P ＜ 0. 05) ，NK cell activity significantly reduce ( P ＜ 0. 05) ． Conclusion Blood parameters and immune function indicators were measured in H22 tumorbearing mice． We provide the basic data for biological response modifiers anticancer drug screening．

Key words: H22 liver cancer, solid tumors, immune function indicators, cyclophosphamide