关键词: 豚鼠, 高钾血症, 治疗

Abstract: Objetive To establish the hyperkalemia model of guinea pig and to compare the effectiveness between conventional therapy and vagus nerve stimulating therapy ;and to observe activity changes of Na-K-ATPase in myocardium and liver under hyperkalemia conditions,in order to make further research on the mechanism of the hyperkalemia, as a basis of the establishment of experiment for undergraduate students. Methods KCl aq was separately injected through abdominal cavity to make hyperkalemia and the changes of ECG were observed with the biological signal gathering analysis system type BL-420F； ion concentration was recorded by biochemical analyzer; ELISA Kits were used to determine activities of Na-K-ATPase. Results After injecting KCl, potassium content in plasma increased,，Na-KATPase activity increased in myocardium and liver. Meanwhile, P waves lowered, T waves tented, QRS complex widened, PR intervals prolongaed and QT intervals shortened Therapies attenuated all these index , especially the glucose and insulin therapy group. Conclusion: injection of KCl aq can successfully produce the model of hyperkalemia and glucose/insulin is the best therapy in the therapies we used, in addition, stimulating vagus nerve can effectively reduce the serum potassium concentration. The results of Na-KATPase suggest that promote the postassium into cells is the basic of hyperkalemia therapy.

Key words: Hyperkalemia, Therapy, Guinea pig