关键词: 生化标记, 实验大鼠, 实验小鼠, 国家标准

Abstract: Abstract： Objective To investigate and evaluate the effects of the two anesthetic methods，sodium pentobarbital plus isoflurane anesthesia and sodium pentobarbital intravenous anesthesia，in experiments of femur fracture in dogs．Methods Ten dogs were equally divided into A and B groups．After induction of anesthesia by sodium pentobarbital．group A was boosted with 0．8％isoflurane to maintain anesthesia status．Group B was given intravenous sodium pentobarbital anesthesia．During anesthesia，heart rate，blood pressure，oxygen saturation and body temperature were monitored at different time points．The data were analyzed using the SPSS 1 3．0．Results Before and after receiving inhalation anesthesia，group A showed significant difference(P<0．05)in heart rate (HR)，oxygen saturation(SP02)，and body temperature(TPR)after 5 min of treatment．Physiological indexes of the dogs were stable and recovery was fast．In the pentobarbital sodium treated group，only the body temperature (TPR)was significantly different(P<0．05)pre-and post—anesthesia．At 45—60min post anesthesia，the dogs resumed eyelid reflex and muscle tremors，so additional pentobarbital sodium was required．Conclusion The inhalation anesthesia is efficient，acts stably，and makes the recovery fast．

Key words: Dogs, Femur fracture, Isoflurane, Pentobarbital, Physiological index