关键词: 无毛小鼠, 细胞免疫, 体液免疫

Abstract: The mutant gene of BALB/c mutant hairless mice was assigned to chromosome 11, Genetic markers which have been tested suggested that the mutation is a new genetic locus that affected the skin and hair structure of the mouse .The mutation was named uncoved,with the symbol Uncv . Uncv has been accepted by International Naming Committee of Mice,and the gene information of Uncv has been receipted by the mice's genebank .The skin is the biggest organ of the body , and is also the main physiological barrier between vivo and vitro .The skin is albe to produce and keep the part immunoresponses , inflammation . Many immunoresponses are related to the skin .The dissecting and breeding of the mutant mice show that their immune organs are normal and they can adopt to the general conditions.Objective To further study the mutant gene's immune function of the BALB/c mutant hairless mice . Method\ We tested the parameters of the immune system about two day old、two month old hairless mice and mutant sparse coat mice . After testing the parameters of CD4+ 、CD3 + 、CD8 + 、CD19 + through flow cytometry and testing the IgG by ELISA.Results\ we found that CD19 + 、CD4 +/CD8 + of male are higher than female about two week old mutant mice with sparse coat , but CD8 + of female is higher than the male,CD4 +/CD8 + of male F 2 sparse coat mice is higher than the female, CD19 + 、IgG of male mutant hairless mice is higher than the female . There were no significant differences among the male and the female about the two month old mutant hairless mice and the mutant sparse coat mice, CD4+ of male F 2 two month old mutant hairless mice is higher than the female . Otherwise , we also found that the celluar immunity and humoral immunity of both two week old and two month old hairless mice are lower than the mutant sparse coat mice ;only the celluar immunity of F 2 mutant hairless mice is lower than the mutant sparse coat mice , but the humoral immunity is higher .The experiment suggest that the mutation of mutant hairless mice can down regulate the mice's immunity .Our studies showed that the mutant gene of BALB/c hairless mice affected the immune function.

Key words: Hairless mice, Celluar Immunity, Humoral immunity