关键词: 家蚕, 生物反应器, 实验动物化

Abstract: By using the domestic silkworm as the bioreactor (reorganizing the nucleus polygon virus expression system) to produce genetic products, we can attain our goal with less cost, higher productivity and activity in a safe manner. With the swelling development of biosciences and biotechnologies in recent years, we should develop new experimental species, especially the lower species, to replace the mammals and explore the animals' potential as a resource to benefit the human beings. We will make a detailed account of what we have achieved by utilizing the domestic silkworm exotic genes and the progress we have made in its animalization breeding technologies. Our domestic silkworm expression system is composed of its cells, transferring carrier, modified BmNPV, its chrysalis. We have obtained the rsj14NPV, which contains Japanese schistosome fatty acid albumen genes, and rsj28NPV, which contains Japanese schistosome 8KDGST genes. After being infected by the rsj14NPV, every silkworm (871×872) can produce 3.5 mgs' purified albumen. After the silkworm 57A.57B×24.26 is infected with Rsj28NPV, every silkworm will turn out 5mgs' purified albumen. By employing rSj14NPV(871×872),we observe the relations between the volume of contracted virus, the timing of the contraction, the duration of the virus and the amount of the produced albumen. The results are as follows: the silkworm that is inoculated with 4μL and 6μL produces 4-5mgs/silkworm, which is the highest, while the silkworm that is inoculated with 1μL group and 2μL group turns out only 1 mg, which is the lowest; the timing of the inoculation has no significant influence on the productivity; the reorganized virus that is preserved for a long period of time(9 months)is not very productive after being inoculated, which shows that we should use the required resurrected reorganized virus to achieve high productivity; by inoculating 55μLs’ resurrected reorganized virus(rsj14NPV) into the silkworm 871×872 will produce 4-5 mgs' albumen, which is similar to that of autumn wind ×white jade. In accordance with the current know how of sericiculture, we establish the common level silkworm breeding technical system, and set down the corresponding technical rules and disease tackling principles. The common level silkworm breeding technical system is composed of 6 technical links, that is, hatching hastening of the eggs; the picking and storing of mulberry leaves; breeding criteria of the 1 3 instar young silkworms; breeding criteria of the 4 5 instar silkworms; driving the silkworm onto a pile of straws to spin; antisepsis and preventing illness. We have confected the man made feeding stuffs for silkworms of all instars and the research on the feeding of the silkworm barrier system silkworm is still in progress. In the research, we will establish a technical system that can control the breeding environments and avoid pathogenic pollutions and the use of antibiotics. It can also carry out recycling breeding so as to meet the needs of silkworms of all kinds.

Key words: Domestic silkworms, Bioreactors, Animalization of the experiment