小鼠肺炎病毒的分离和鉴定

实验动物科学

小鼠肺炎病毒的分离和鉴定

吴惠英;雷文绪;王秀清;刘媛;

中国药品生物制品检定所实验动物标准化室,中国药品生物制品检定所实验动物标准化室,中国药品生物制品检定所实验动物标准化室,北京市实验动物研究中心北京天坛西门100050,北京天坛西门100050,北京天坛西门100050,北京天坛西门100050

收稿日期:1992-03-25 修回日期:1992-03-25 出版日期:1992-03-25 发布日期:1992-03-25

IDENTICATION OF PNEUMONIA VIRUS OF MICE AND ASSASY OF IT'S ANTIBODY IN EXPERIMANT MICE

WU HUI-YING, LEI WEN-XU, WANG XIU-QING, LIU YUAN National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050

Received:1992-03-25 Revised:1992-03-25 Online:1992-03-25 Published:1992-03-25

摘要/Abstract

摘要： 本文采用SPF小鼠滴鼻分离到一株鼠肺炎病毒,经鸡胚试验、组织培养、间接免疫荧光试验(IFA),抗体产生实验,同时用美国鼠肺炎(PVM)药盒的验证试验均证实了该分离物为鼠肺炎病毒(简称PVM-Bj)。1986年PVM抗体检测按季度统计,其阳性率分别为51％,15％,7.7％,0％。

关键词: 鼠肺炎病毒(PVM), 分离物的鉴定, PVM抗体测定

Abstract: Since 1980 the Pneumonia Virus of Mice(PVM)strain has been isolated form mice feeding in our farm and propagated with chick enbryo. The culture could led to paghological changes. IF test of freeze section of lung ang antibody production test in infected SPF mice was performed with U.S. reagent kit as control. The results shawed that the culture is PVM Strain, and called PVM-BJ. From 1985-1988 the antiboby in expermental mice was tested and such as the positive rate of mouse antibody reached 52％ in 1986.

Key words: PVM, laolation, ldentification

吴惠英;雷文绪;王秀清;刘媛;. 小鼠肺炎病毒的分离和鉴定[J]. 实验动物科学.

WU HUI-YING, LEI WEN-XU, WANG XIU-QING, LIU YUAN National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050. IDENTICATION OF PNEUMONIA VIRUS OF MICE AND ASSASY OF IT'S ANTIBODY IN EXPERIMANT MICE[J]. laboratory animal science.