关键词: 高脂血症, 骨质疏松症, 大鼠模型, 联合诱导

Abstract: Objective　This project aims to establish a rat model of osteoporosis combined with hyperlipidemia, providing a basis for selecting animal disease models for future studies on the pathogenesis and therapeutic efficacy of osteoporosis complicated with hyperlipidemia.Methods　After one week of adaptive feeding, 12 rats were randomly divided into a control group with 6 rats and an experimental group with 6 rats. The control group was given regular feed, while the experimental group was given 60% high-fat feed plus intramuscular injection of dexamethasone (5 mg/kg, twice a week), after 13 weeks of modeling, samples were taken. Calculate liver coefficient and body fat percentage.The fully automated biochemical analyzer detects serum levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL); part of the liver tissue was harvested for HE staining and observation of its pathological morphology, and the right femurs were dissected for bone density examination.Results　Compared with the control group, the liver coefficient and body fat percentage of rats in the experimental group were significantly increased (P<0.01 and P< 0.01, respectively).The levels of TG, TC, HDL, and LDL were significantly increased (P<0.05, respectively). The surface color of the liver turns yellow, the edges become dull, and the texture is softer; the arrangement of rat liver cells is disordered, with a significant increase in volume and extensive steatosis. There are large lipid droplets and vacuoles in the cytoplasm, and some nuclei are compressed and pushed to the edge of the cell.The bone density content in the right R1, right R2, and all right regions significantly decreased (P<0.001, respectively).Conclusion　A rat model of osteoporosis combined with hyperlipidemia can be successfully established by feeding with high-fat diet and administering dexamethasone via intramuscular injection for 13 weeks.

Key words: hyperlipidemia, osteoporosis, rat model, combined induction