关键词: 哨兵小鼠, 屏障环境, IVC 系统, 寄生虫感染

Abstract: Objective To investigate the effective time and sensitivity of sentinel mice in monitoring mite and flagellate infection in IVC System in barrier environment under different types of soiled bedding ( corncob and shavings) , and to explore the actual monitoring ability of sentinel mice. Method 42 sentinel mice ( 4-week-old female ICR) were randomly divided into 6 groups. The sentinel mice were raised with 100% of the feeding soiled bedding ( corncob or shavings) from mite and flagellate double positive mice, or directly placed in the cage of the mice infected with both mites and flagellates. Then the sentinel mice were detected at different times after exposure. Result After 6 weeks, the mite infection rates of the sentinel mice added soiled corncob and shavings bedding were 20% and 60%, respectively, but the detection of flagellate in both groups was negative. After 8 weeks, the mite infection rate of sentinel mice with 100% soiled corncob bedding was 33. 33%, and the flagellate infection rate was 16. 67%; the mite infection rate of sentinel mice with 100% soiled shaving bedding was 83. 33% and no flagellate was detected. The sentinel mice, which were directly raised with mite and flagellate double infected mice in the same cage, were 100% infected with mites and flagellates on day 25. Conclusion The corncob soiled bedding is less sensitive than shavings soiled bedding in monitoring mite infection, suggesting that shavings soiled bedding exposure method has reference significance for mice whose population is not easily expanded.

Key words: sentinel mice, barrier environment, IVC system, parasitic infection