关键词: 肠梗阻, 肠绞窄, 动物模型

Abstract: Objective To establish a rat model of strangulated intestinal obstruction,which can present good time-dependent relation and unification between the intestinal incarceration time and the degree of intestinal necrosis. Methods By choosing adult healthy male Spraque-Dawley rats,an incarceration hernia model to imitate the strangulated intestinal obstruction was made. In the incarceration hernia model,a midline fascial defect was made,the length of the defect was set by the 80 percent of the diameter of incarceration bowel,and the length of incarceration bowel was 6 cm. All rats were divided into six groups by different intestinal incarceration time,from 1 hour to 6 hours step by 1 hour. With the sham operation group as the contral group,the grade of intestinal injury between the groups was compared by necrosis degrees under microscopy,and the statistic method was ritid analysis. Results Comparing with the control group,the different degree of intestinal necrosis was found,the longer intestinal incarceration time and the higher degree of intestinal necrosis. But with the same intestinal incarceration time,the degree of intestinal necrosis was similar. The mean ritid value of control group was 0. 038; the mean ritid value of each experimental group from 1 hour to 6 hours was 0. 248,0. 329,0. 464,0. 551,0. 765 and 0. 874,respectively. Using LSD test of analysis of variance,microscopic intestinal injury in each eaperimental group was significantly different from the control group(P < 0. 035); the significantly difference of intestinal injury was found between the experimental groups,which difference of intestinal incarceration time was 2 hours or more(P > 0. 09); no significantly difference of intestinal injury was found between the experiment groups,which difference of intestinal incarceration time was 1 hours,except for the 4 hours group and 5 hours group(P = 0. 01). Conclusion A rat model of strangulated intestinal obstruction has been established,which can present good time-dependent relation and unification between the intestinal incarceration time and the degree of intestinal injury.

Key words: Intestinal obstruction, Strangulation, Animal model