关键词: 生化指标, 胰岛素抵抗

Abstract: Objective To analyze the effect of serum biochemical indicator and insulin resistance in SD rats induced by four types fatty acids: medium-chain saturated fatty acids (MC-SFA, MCF group), long-chain saturated fatty acids (LC-SFA, LCF group), n-6 polyunsaturated fatty acids (n-6 PUFA, SUF group), and n-3 polyunsaturated fatty acids (n-3 PUFA, TUF group). Methods Normal male SD rats were fed on standard laboratory chow (8.23% of calories as fat) or isocaloric high fat diets (44.75% of calories as fat) for 10 weeks. Serum glucose (GLU), insulin, triglyceride (TG), cholesterol (CHO) and HDL-cholesterol (HDL-c) were measured with an interval of 18 days. The insulin sensitivity was estimated by ISI index (ISI= ln 1/( FPG×FINS)). Results After 10 weeks, body weights of LCF and SUF group were obviously higher than those of normal and other high-fat groups. Serum TG was obviously higher in LCF group compared with other high-fat groups P<0.05). High-fat feeding groups, especially the TUF group, exhibited obviously lower HDL-c (P<0.05) than normal group; Serum insulin significantly increased in LCF group compared to other groups (P<0.05); Both LCF and TUF group showed lower ISI than normal group (P<0.05); However there was no significant difference in GLU among the five groups. Conclusion Body weight, serum biochemical indicator and insulin resistance of rats are affected significantly by the types of fatty acids.

Key words: Biochemical indicator, Insulin resistance, SD rats