关键词:

Abstract: To investigate the effects of IVF reagents on the fertility rate and birth rate of
genetically modified mice in biological purification. Method The experimental system was divided into
group A, group B and group C according to different manufacturers of IVF reagents. 5 - 11 genetically
modified mouse strains were selected for superovulation, IVF-ET experiments, and the number of eggs
collected, the number of 2-cell, the number of embryos transferred, and the number of piglets born were
recorded, and the fertilization rate and the birth rate were calculated. Result The fertilization rate of
group A was ( 51. 89 ± 13. 91 ) % and the puerperal rate was ( 14. 70 ± 10. 95 ) %. In group B, the
fertilization rate was ( 57. 71 ± 17. 12) % and the birth rate was ( 34. 96 ± 9. 92 ) %. In group C, the
fertilization rate was (54. 78±18. 56) % and the puerperal rate was (22. 01±9. 54) %. Fertilization rate:
B>C>A; Puerperal rate: B>C>A, but there was no statistical difference among all groups. The reagents
required by group A are difficult to prepare, complicated to use and have poor stability, while the
reagents of group B and group C are simple to use and have strong stability. However, compared with group A and group C, the unit price of reagents used in group B is high, so the experimental cost is
higher. Conclusion The reagents of group B and group C can be used as conventional reagents for IVF
of genetically modified mice, and the experimental can choose them according to the comprehensive
factors such as fertilization rate, puerperal rate, price and ease of use.

Key words: genetically modified mice, assisted reproduction, biological purification, in vitro fertilization
( IVF) , fertilization rate, farrowing rate