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Abstract: Differential analysis of intestinal flora function in guide dogs and eliminated dogs
was performed using macro gene sequencing. Method Fifteen dogs were sequenced ( nine guide dogs
and six eliminated dogs) and analyzed by applying gene number and intestinal flora diversity. Result
There were 25 630 genes specific to the guide dog group and 10 527 genes specific to the elimination dog
group. There were 2 266 up-regulated genes and 1 043 down-regulated genes with significant differences
(P<0. 05) . The abundance of Clostridium purpureum was higher in the guide dog group than in the
elimination dog group; in addition, the abundance of rumenococci differed between the two groups of dog
samples. Conclusion The diversity of intestinal flora of both guide dogs and eliminated dogs was
different and had an effect on dog behavior.

Key words: gut microbiota, metagenomics sequencing, microbiota analysis