关键词: 顺铂, 耳毒性, 丹参素钠, 活性氧

Abstract: :Objective To investigate the inhibitory effect of Sodium Danshensu ( SDSS) on ototoxicity of cisplatin. Method HEI-OC1 cell lines were selected to construct cisplatin-induced damage models based on which protective agents were screened in the small-molecule compound library and Determination of IC50 of cisplatin in HEI-OC1 cells at 24 h. The effect of SDSS on hair cell survival was measured in HEIOC1 cell models and cisplatin-induced mouse cochlear implant models. The effect of SDSS on cisplatininduced oxidative stress levels in HEI-OC1 cells was measured by superoxide anion fluorescence probe (DHE) staining and the positive control was NAC. Result SDSS, a potent protective agent, was selected from 210 FDA-approved small molecule compounds, and SDSS pretreatment significantly inhibited cisplatin-induced decrease in HEI-OC1 cell activity, apoptosis and reactive oxygen species (ROS) aggregation at an optimal concentration of 200 μmol / L. In addition, SDSS significantly inhibits cisplatin-induced damage to hair cells in mouse cochlear explants. Conclusion SDSS may play a protective role in cisplatin-induced ototoxicity by inhibiting ROS aggregation and apoptosis in hair cells.

Key words: cisplatin, ototoxicity, SDSS, reactive oxygen species