关键词: C57BL/6小鼠, 亚系, 遗传差异, 表型差异, 遗传质控

Abstract: The C57BL/6 mouse is the most widely used experimental rodent to date. C57BL/6J and C57BL/6N represent the major lineages of C57BL/6 mouse, from which a large number of substrains have been derived following long-term independent breeding. Genetic background differences among substrains are likely to cause phenotypic divergence, which directly affects the reliability and reproducibility of experimental result. If C57BL/6 substrains with unclear genetic backgrounds are used in experiments, potential genetic differences may cause unpredictable interference to the research result. Therefore, institutions responsible for breeding C57BL/6 mouse colonies should prioritize and implement genetic quality control. Scientific measures should be taken to promptly identify genetic contamination or substrain admixture, maintain the stable and well-defined genetic background of the substrains, and eliminate interference with research caused by mixed genetic backgrounds at the source.

Key words: C57BL/6 mouse, substrain, genetic differences, phenotypic differences, genetic quality control