关键词: 刚毛棉鼠, 转录组测序, 基因表达, 基因功能注释

Abstract: Objective　To investigate the differences in gene expression in different tissues of Sigmodon hispidus, and promote the open sharing of laboratory animal resource.Methods　RNA-seq sequencing technology was used to perform transcriptome sequenceing and bioinformatics analysis on five tissues (heart, liver, spleen, lungs, and kidney) of Sigmodon hispidus. Results　After quality control processing, a total of 461 666 539 sequences were obtained from 20 samples, with the base number ranging from 6.37 G to 7.99 G for each sample. The clean reads were assembled using Trinity software to obtain 216 139 unigenes with an average length of 1 170 bp. A total of 168 458(77.93%) unigenes were successfully annotated in seven databases, including 26 394 (12.21%) and 38 056 (17.60%) unigenes annotated in KEGG and GO databases, respectively. Through further analysis of the gene expression levels between different tissues, found that the number of uniquely expressed genes in the heart, liver, spleen, lung, and kidney were 12 297, 14 477, 35 104, 23 728, and 9 636, respectively. And a total of 103 359 differentially expressed genes between different tissues was detected.Conclusion　This study obtained gene expression data of different tissues of Sigmodon hispidus,through transcriptome sequencing analysis, providing reference for further research on the biological characteristics of Sigmodon hispidus and the development and utilization of functional gene resources of this laboratory animal.

Key words: Sigmodon hispidus, RNA-seq sequencing, gene expression, gene function annotation