关键词: 眼眶静脉丛采血, 下颌静脉采血, 尾尖采血, 血红蛋白, 皮质酮, 促肾上腺皮质激素 

Abstract: Objective To evaluate the influence of different blood collection method on the quality of blood samples in mice. Method For C57BL / 6J and ICR mice, three blood collection sites were used: orbital venous plexus, mandibular vein and caudal tip, and blood samples were collected at anesthetic or not, and serum and plasma were separated, respectively. The light absorption of samples at 415, 541 and 576 nm was measured to evaluate the hemolytic quality. ELISA was used to measure corticosterone and adrenocorticotropic hormone to evaluate the stress states of animals. Result The absorbance variability of the blood samples obtained by the tail tip blood collection method at 415 nm, 541 nm and 576 nm was large, and the heme light absorption of some samples was high, which was statistically different from the other two method. There was no significant difference in the level of adrenocorticotropic hormone in serum and plasma samples obtained by different blood collection method, and the plasma corticosterone content obtained by the orbital venous plexus collection method of non-anesthetized animals was higher than that obtained by the mandibular blood collection method( P < 0. 05) . Conclusion The blood samples from the tail tip are affected by the stability of operation, and there is a risk of hemolysis in blood samples. Mandibular blood collection is easy to operate, the collection volume is large, the blood collection time is short, the hemolysis risk is low and the level of irritation to animals is low than method by orbital venous plexus. 

Key words: blood from the orbital venous plexus, mandibular vein blood drawing, caudal tip blood retrieval, hemoglobin, corticosterone, adrenocorticotropic hormone