关键词: 缺氧损伤, 利舒康胶囊, 心肌组织, Nrf2 / HO-1 通路

Abstract: Objective To study the protective effect and mechanism of Lishukang capsule on myocardial tissue induced by hypoxia at simulated altitude in rats. Method Sixty male Wistar rats were randomly divided into normal control group, hypoxia model group, positive rhodiola capsule group (420 mg / kg) , and different concentrations of Lishukang capsule groups (280, 420, 560 mg / kg) , with 10 rats in each group. Normal control group and hypoxia model group were given equal volume of water for injection for 7 consecutive days. 50 min after the 7th day of administration, except for the normal control group, all groups were put into large low-pressure and hypoxic animal laboratory chamber to simulate the altitude of 8 000 m and hypoxia for 12 h. After 12 h, the rats were sacrificed by lowering the altitude to 3 500 m, and the myocardial tissue was taken for ultrastructural observation. The expression changes of Nrf2 and HO-1 in cytoplasm and Nrf2 in nucleus were detected by Western blot. Result Compared with the normal control group, the mitochondrial outer membrane of myocardial cells in hypoxia model group was destroyed, obviously swelling, serious myocardial injury, and the expression of Nrf2 and HO-1 in tissues increased (P<0. 05 or P<0. 01) ; Compared with the hypoxia model group, the myocardial injury in the rhodiola capsule group and rishokang high-dose group was significantly improved, with intact myofilm and mitochondrial structure, and the expression of Nrf2 and HO-1 decreased ( P < 0. 05 ) , there was a decreasing trend in risocan low-dose, medium-dose and high-dose groups, and there was a significant difference in risocan high-dose groups (P<0. 05) . Conclusion Lishukang capsule has protective effect on myocardial injury caused by altitude hypoxia, and its mechanism may be related to the activation of Nrf2 / HO-1 signaling pathway.

Key words: plateau hypoxia, Lishukang capsule, myocardial tissue, Nrf2 / HO-1 pathway