关键词: 党参多糖, 脑缺血再灌注, S100-β 蛋白, 神经元特异性烯醇化酶, 大鼠

Abstract: Objective To observe Codonopsis pilosula polysaccharide ( CPPS) on ischemia-reperfusion injury of rat serum S100-β protein and neuron-specific enolase ( NSE) levels. Method SD rats were randomly divided into 5 groups, namely sham operation group, model group, Edaravone group and 2 codonopsis pilosula polysaccharide (CPPS) high-dose and low-dose groups, with 10 rats in each group. By using line switch block rat middle cerebral artery occlusion ( MCAO) 2 h reperfusion 24 h after cerebral ischemia reperfusion model is set up, two CPPS and edaravone group rats were given corresponding drugs for 14 consecutive days before surgery, Nissl staining and HE were used for histological observation 24 h after reperfusion. Cerebral water content was measured and Evans blue method was used to assess cerebral apoplexy. Serum NSE and S100-β levels were determined by ELISA. Result Compared with sham operation group, the water content and permeability of brain tissue in model group were significantly increased ( P < 0. 01) , and the contents of NSE and S100-β protein in serum were significantly increased ( P < 0. 01 ) . Compared with model group, serum NSE and S100-β protein contents in CPPS high-dose and low-dose groups were significantly decreased ( P < 0. 01) , and brain tissue water content and permeability in CPPS high-dose group were significantly decreased ( P < 0. 01) . Conclusion CPPS can significantly reduce the level of NSE and S100-β in serum of rats with cerebral ischemia-reperfusion injury, and have good neuroprotective effect.

Key words: codonopsis pilosula polysaccharide(CPPS) , cerebral ischemia / reperfusion, S100-β protein, neuron specific enolase(NSE) , rats