关键词: 高脂血症模型, 高脂饲料, SD 大鼠, 胆固醇

Abstract: Objective To discover the hypolipidemic drugs, we established the model of hyperlipidemia in Sprague-Dawley ( SD) rats by feeding the high-fat diets of different recipes. Method Male SD rats were randomly assigned to 6 groups:the control group and 5 model groups. The rats were fed with the control diet and the high-fat diet of five different recipes for 6 weeks respectively. The concentrations of serum lipids were detected at the end of 1, 2, 4 and 6 weeks ’ feeding. The hepatic lipids ’ concentrations were determined at the end of 6 weeks. Result Compared with the control group, after 6 weeks of feeding, the concentrations of serum total cholesterol ( TC) of the rats in model group 2 and 3 significantly increased; the concentrations of serum low-density lipoprotein cholesterol ( LDL-C) of the rats in all model groups significantly increased. And the hepatic lipids ’ concentrations significantly increased in all model groups. Conclusion The model of hypercholesteremia was established by feeding the high-fat diet of recipe 2 and 3 that adding 20% fructose and 10% lard for 6 weeks in rats. this experiment provides a simple, low-cost, and stable animal model for the development of lipid-lowering drugs.

Key words: hyperlipidemia model, high-fat diet, Sprague-Dawley rats, cholesterol