关键词: 姜黄素, 癫痫, 小胶质细胞, 神经炎性反应 

Abstract: Objective To explore the effect and mechanism of curcumin on seizures in mice. Method 60 C57 / BL6 male mice were randomly divided into normal group, epilepsy model group and curcumin treatment group, with 20 mice in each group. Racine grade score of seizures and the latency of seizures in each group were observed and recorded. The expressions of GFAP and IBA-1 in hippocampal CA3 region were detected by immunofluorescence staining, and the expression levels of hippocampal IL-1β, NLRP3, caspase1 were detected by Western blot. Result Racine score of seizures in curcumin treatment group was significantly lower than that in epilepsy model group(P< 0. 05) , and the seizure latency was significantly prolonged ( P < 0. 05 ) . Compared with the control group, the expression of GFAP and IBA-1 in hippocampal CA3 region in the model group was significantly increased ( P < 0. 01) , glial cells were significantly activated, and the expression levels of hippocampal IL-1β, NLRP3, caspase1 were upregulated significantly ( P < 0. 01 ) . Compared with the model group, the number of GFAP and IBA-1 positive cells in hippocampal CA3 of curcumin group were decreased dramatically( P< 0. 05) , and the expression levels of hippocampal IL-1β, NLRP3, caspase1 were decreased notably ( P < 0. 05) . Conclusion Curcumin can reduce the severity of seizures, which may be through the regulation of NLRP3 / caspase1 to inhibit hippocampal inflammation. 

Key words: curcumin, epilepsy, microglia, neuroinflammation