关键词: 胃癌, IL-6 / JAK2 / STAT3 信号通路, 增殖

Abstract: Objective To study the effect of IL-6 / JAK2 / STAT3 signaling pathway on the proliferation of gastric cancer. Method MFC gastric cancer tumor-bearing mice models were established and randomly divided into model group,AG490 blocker group, and Stattic blocker group, each with 12 mice, and another 12 healthy mice were selected as the blank group. After the intervention,the tumor tissue was taken to test the weight and calculate tumor inhibition rate;ELISA method to detect IL-6 content in mouse serum; Immunohistochemical method was used to detect IL-6,JAK2,STAT3,c-Myc and Cyclin D1 protein expression in tumor tissues;Western blot was used to detect IL-6,JAK2,p-JAK2,STAT3,p-STAT3,c-Myc and Cyclin D1 protein expression in tumor tissues;RT-qPCR method to detect IL-6,JAK2,STAT3,c-Myc and Cyclin D1 mRNA expression in tumor tissues. Result Compared with the control group,the IL-6 content in the serum of the model group was increased (P<0. 01) ;Compared with the model group, serum IL-6 content,tumor mass,the protein content of IL-6,STAT3,p-JAK2,p-STAT3,c-Myc and Cyclin D1,and the mRNA content of IL-6, JAK2, STAT3, c-Myc and Cyclin D1 decreased ( P < 0. 05, P < 0. 01 ) . Conclusion Blocking the IL-6 / JAK2 / STAT3 signaling pathway can affect the expression of downstream target proteins,thereby inhibiting the proliferation of gastric cancer. 

Key words: gastric cancer, IL-6 / JAK2 / STAT3 signaling pathway, proliferation