关键词: 槐芩软膏, 痔疮, 肛缘水肿, 创面愈合, NF-kB

Abstract: Objective To explore the effect and potential mechanism of Huaiqin ointment on perianal edema and wound healing in mouse model with hemorrhoids. Method 30 ICR mice were used to establish the mouse model with hemorrhoids via injecting acetic acid to perianal region, then mice were divided into control group and treatment group randomly. Mice in control group were treated with low concentration potassium permanganate cleaning, and mice in treatment were treated with low concentration potassium permanganate cleaning plus applying Huaiqin ointment. After 7 days treatment, the degree of perianal edema and wound healing in perianal region was analyzed. The hs-CRP, IL-6, TNF-α, hemoglobin levels in plasma or perianal tissues were measured by ELISA or qPCR. HE staining and immunofluorescence were used to detect the inflammatory cell infiltration in perianal tissues. Western blot was used to measure the phosphorylation of IkBα and intranuclear NF-kB p65 contents in perianal tissues. Result The degree of perianal edema, wound healing, the blood hs-CRP, the cytokines ( IL-6, TNF-α) in blood or perianal tissues, hemoglobin in perianal tissues of treatment group ameliorated significantly compared to control (P<0. 05) . HE staining and immunofluorescence showed that the inflammatory cell infiltration in perianal tissues in treatment group was significantly fewer than control group ( P<0. 05) . Western blot showed that the IkBα phosphorylation and content of p65 in nuclear of treatment group was also significantly lower than control ( P < 0. 05 ) . Conclusion Huaiqin Ointment treatment attenuates inflammation response and perianal edema, promotes wound healing, of which mechanism is involved in NF-kB signal pathway.

Key words: Huaiqin ointment, hemorrhoids, perianal edema, wound healing, NF-kB