关键词:  , GalR2, 基因敲除, 小鼠, 繁育, PCR

Abstract: Objective To breed and culture galanin receptor 2 knockout mice, and mouse genotypes were analyzed and identified, which was also verified for the applicability of the detection method. Method F1 generation were obtained via GalR2 knockout heterozygotes male mice and wild-type female mice bred at 1 ∶ 2 ratio, or heterozygous female mice and wild-type male mice bred at 1 ∶ 1 ratio. The obtained F1 generation heterozygous mice were bred for sibling mating to obtain F2 generation homozygous, heterozygous and wild-type mice. The tails (2-5 mm) of 2- week-old mice were cut to obtain genomic DNA, PCR was utilized to amplify the target gene fragments and genotype result were determined by agarose gel electrophoresis. Result The breeding and reproducing were successful with a number of knockout mice were stably bred. Three genotype offspring of F1 generation including homozygous ( GalR2- / - ) , heterozygous ( GalR2+ / - ) and wild-type ( GalR2+ / + ) were identified using the PCR method. Conclusion GalR2 knockout mice can be bred and cultured in a scientific way and genotypes can be identified with PCR method.

Key words: GalR2, gene knockout, mice, breeding, PCR