关键词: Hepa1-6

Abstract: Objective To develop a murine subcutaneous transplantation model of hepatoma with cell line Hepa1-6.Methods Hepa1-6 cell was cultured in vitro and its growth was monitored;Hepa1-6 cells were injected intradermally into C57BL/6J mice at concentrations of 2×10~5/mice(A group),2×10~6/mice(B group)and 4×10~6/mice(C group).Results The cells grew very slowly,and they went into exponential growth three days after assimilation;the rate of tumorigenesis in group A,B and C was 30%,80% and 100% respectively;Tumorigenesis was observed after 12 and 10 days in group A and B respectively,but after 7 days in group C;From the third to the forth week,the tumor bulks in group C and B grew significantly bigger than those in group A(P<0.01). There was no obvious difference in tumor bulks between Group C and B.Conclusion The murine hepatoma model can be established by subcutaneous transplantation of Hepa1-6 cells at the concentration of 4×10~6/mice.

Key words: Hepa1-6, Subcutaneous transplantation model of hepatoma