关键词: 骨缺损模型, 皮下浅筋膜, 负载材料, 微型计算机断层扫描技术

Abstract: Objective To develop a bone defect model suitable for loading degradable materials. Method A rat femoral midshaft segmental bone defect model was established. Subcutaneous superficial fascia ( 1 cm×2 cm) was harvested from the surgical site, and a 1. 2 mm-diameter bone traction pin was used to fix the femur. A 4-mm-long segment of half-bone tissue was excised from the midshaft to create the bone defect model. The defect area was wrapped and sutured with the superficial fascia, followed by layered suturing of the muscle and skin. The experiment was divided into three groups: ( 1) the blank control group, which received no fascial wrapping; ( 2) the inactivated fascia group, in which the defect area was wrapped with superficial fascia inactivated by 70% ethanol for 10 min; ( 3 ) the fascia-wrapped group, in which the defect area was wrapped with fresh superficial fascia. Result Compared with the blank control group, both the inactivated fascia group and the fascia-wrapped group showed significantly accelerated bone defect healing. The X-ray scores were significantly higher in the inactivated fascia group and the fascia-wrapped group than in the blank control group (P<0. 05) , and the percentage of healing area was significantly higher than that in the blank control group ( P<0. 01) . The fascia-wrapped group exhibited the best healing effect. Conclusion The improved bone defect model significantly enhances bone defect healing and shortens the healing time, providing a more suitable animal model for loading degradable materials.

Key words: bone defect model, subcutaneous superficial fascia, loadable materials, Micro-computed tomography (Micro-CT)