关键词: 支气管哮喘, 特异性免疫, 炎性反应, 辅助性 T 细胞 1, 辅助性 T 细胞 2

Abstract: Objective To explore the effect of specific immunity on inflammation in bronchoalveolarlavage fluid ( BALF) of asthmatic mice by analyzing Th1 / Th2 expression. Method Forty mice weredivided into control group, model group, SIT group, and dexamethasone group, with 10 mice in eachgroup. Except for the control group, asthma mouse models were established in the other groups. After thestimulation phase, the SIT group was injected with 1 mg of OVA, the dexamethasone group was given a0. 075 mg / mL solution, and the remaining rats were injected with an equal volume of PBS solution. Thelung function meter was used to detect airway responsiveness; the BALF inflammatory cells were observedby Wright-Giemsa staining; ELISA was used to detect cytokines IL-13, IL-4, IL-5, and INF-γ in BALF;HE staining was used to observe lung tissue morphology; PAS staining was used to observe cup-shapedcell activity in lung airways; and flow cytometry was used to detect the proportion of Th1 and Th2 in lungtissues. Result Specific immunity significantly reduced airway hyperreactivity in model mice, reducedinflammatory cell activity in BALF, and inhibited asthma occurrence and development by increasing Th1实验动物科学 41 卷while decreasing Th2. Conclusion Specific immunity can alleviate airway hyperresponsiveness andinhibit inflammation in asthmatic mice, improve cup-shaped cell proliferation, and relieve ventilation,possibly related to regulating Th1 / Th2 expression.

Key words:  bronchial asthma, specific immunity, inflammatory response, T-helper 1 cells, T-helper2 cells