关键词: 高尿酸血症, 疾病模型, 尿酸, 时间因素

Abstract: Objective　In order to explore the dynamic changing regularity of each physiological index on acute hyperuricemia model in rats.Methods　The acute hyperuricemia rat model was established by the intraperitoneal injection of hypoxanthine and subcutaneous injection of oteracil potassium in different doses. At 2, 4, 8 and 12 hours after modeling, 6 animals were taken respectively to determine blood uric acid, blood creatinine, serum urea nitrogen and serum. The activity of xanthine oxidase (XOD) in the liver was examined and the renal histopathological test was conducted.Results　Hypoxanthine combined with oteracil potassium can successfully establish hyperuricemia model in rats. Two hours after administration of 100 mg/(kg·bw) hypoxanthine and oteracil potassium, the level of serum uric acid was significantly increased (P<0.05), but the level of serum creatinine and serum urea nitrogen did not increase significantly. However, when the dose of oteracil potassium was increased to 200 mg/(kg·bw), 4 h after modeling, the level of serum creatinine and serum urea nitrogen was increased significantly (P< 0.05). We also found that the degree and duration of the model were direct proportionality to the dose of  hypoxanthine and oteracil potassium, and the levels of serum uric acid, serum creatinine and serum urea nitrogen showed certain dynamic changing regularity. In addition, the pathological damages of kidney were found in each dose group, and the damage scope expanded with time. Finally, in the situation of acute attacks of hyperuricemia, no significant changes of xanthine oxidase (XOD) activity in serum and liver were observed.Conclusion　When establishing acute hyperuricemia model in rats, it may be suitable that the dosage range of hypoxanthine is between 100-300 mg/(kg·bw), and the dosage range of potassium oxazinate dose is between 100-200 mg/(kg·bw).

Key words: hyperuricemia, disease model, uric acid, time factors