关键词: 小鼠, 给药途径, 尾静脉注射, 实验技术与操作

Abstract: Objective　To provide a novel method for tail vein injection in mice, aimed at improving efficiency and success rates.Methods　One hundred and twenty SPF C57BL/6J wild-type mice were randomly divided into four groups:cage cover suppression injection group, fixed lamp light injection group, conventional distal tail vein injection group, and proximal tail vein injection group (experimental group), with 30 mice in each group. Mice were restrained using a tail vein fixator and the tail was secured using a specific technique. Insulin syringes were used for injecting by proximal tail vein. The success rates of various puncture method were recorded and statistically analyzed using SPSS version 27.0.Results　Compared to the methods of restraining with cages, stabilizer with light exposure, and conventional distal tail vein injections, the proximal tail vein injection method demonstrates significantly improved success rates (P<0.05).Conclusion　This approach is accurate, efficient, and convenient, offering a new perspective for tail vein injection in mice.

Key words: mice, drug administration routes, tail vein injection, laboratory techniques and procedures