关键词: 肠道病毒, 野生恒河猴, 年龄, 性别, 系统发育

Abstract:

Objective This study aims to investigate the spectrum of enteric viruses in wild rhesus macaques, providing a scientific basis for the management and introduction of wild monkey populations, disease prevention, and occupational health protection for caregivers. Method This study collected 218 fresh fecal samples of wild rhesus macaques in Yunnan Province, fecal samples from rhesus macaques were collected, viral RNA was extracted, and RT-snPCR detection was performed using enterovirus universal primers 222 / 224 and AN88 / AN89, and positive samples were sequenced and analyzed to confirm the virus type. Result This rhesus monkey group had an enterovirus carrier rate of 11% ( 24 / 218) , as follows: baby monkeys had a carrier rate of 7. 5%(4 / 53) ; young monkeys had a carrier rate of 3. 5% (2 / 57) ; adult monkeys had a carrier rate of 16. 7%(9 / 54) ; and the carrier rate of the old monkey group was 16. 7%( 9 / 54) . Conclusion Genotyping of enteric viruses indicated that the virus spectrum primarily consisted of Enterovirus J, Enterovirus sp. and Enterovirus A groups. Phylogenetic analysis suggested that the virus spectrum is closely related to EVJ, EVA92, EVA119, SV19, and SV46 enteroviruses.

Key words: enterovirus, wild rhesus macaques, age, gender, phylogenetic analysis