关键词: 淋巴瘤荷瘤鼠, 磁共振;活体成像;超声造影;肿瘤血管

Abstract: Objective To investigate the role of three imaging modalities including MRI, in vivo imaging and ultrasound in evaluating chemotherapy in lymphoma-bearing mice. Method 10 BALB / c female mice were subcutaneously injected with lymphoma cells on the right neck and shoulder to build a tumor-bearing model, and were randomly divided into a CTX intervention group and a 0. 9% NS intervention group. Before and after the intervention, MRI, in vivo imaging, and Ultrasound were used to detect tumor-related indicators. After the detection, the mice were sacrificed, and the tumor was taken for volume measurement and pathological examination. Result MRI accurately demonstrated a decrease in tumor size after intervention, which was comparable to gross measurements. In vivo imaging can indicate that the tumor size was reduced and the fluorescence intensity of the tumor decreased after the intervention. Combined with the analysis of the pathological section, the CEUS demonstrated that the perfusion of the tumor-related blood vessels decreased after the intervention, and the differences were statistically significant. Conclusion The three imaging modalities have the advantages of good imaging effect, high imaging sensitivity, non-invasive and reproducible, and the combination of multiple imaging modalities has a better synergistic evaluation effect on the intervention indicators of tumor mouse models.

Key words: lymphoma tumor-bearing mice, MRI, in vivo imaging, CEUS, tumor angiogenesis