关键词: 支气管哮喘, 抑郁, 动物模型, 大鼠

Abstract: Objective To evaluate the reliability of establishing a young rat model of bronchial asthma with depression behavior by solitary cage combined with ovalbumin. Method Young SD rats were randomly divided into control group, solitary control group, asthma group and solitary asthma group. The rat model of asthma with depression behavior was established by solitary cage and gregarious cage feeding combined with ovalbumin-aluminum hydroxide suspension intraperitoneal injection sensitization and ovalbumin normal saline atomization stimulation. The rat’ s weight, physical signs, lung pathology, white blood cell count and classification in venous blood, and serum content of IL-4 BDNF were observed and recorded. The behavior of rats was evaluated by sugar consumption test and forced swimming test. Result Compared with the two control groups, the weight growth of gregarious asthma group was slow ( P < 0. 05) , and the weight growth of solitary asthma group was significantly slow (P<0. 05) ; the gregarious asthma group and solitary asthma group had typical asthma like symptoms such as polypnea, scratching ear nose, the solitary asthma group had depression symptoms such as less activity and less water and food intake; the lung tissue had asthma pathological changes; the white blood cell count and lymphocyte count were increased (P<0. 05) ,compared with the two control groups and gregarious asthma group, IL-4 in serum increased ( P< 0. 05) , BDNF in serum decreased ( P< 0. 05) , sugar preference index decreased (P<0. 05) , immobility time prolonged in forced swimming test (P<0. 05) . Conclusion It is reliable to establish a young rat model of bronchial asthma with depression behavior by solitary cage combined with ovalbumin.

Key words: bronchial asthma, depression, animal model, rat